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FIGURE 4 Relationship between PCDHGA10 protein expression and tumor-infiltrating immune cells and immune checkpoints. (A-C) Multispectral composite of CD3, CD4, CD8, CD11b, CD66b, <t>CD68,</t> CK and DAPI, magnification ×200. (D) Four-color multispectral composite of LAG3, PD1, CK and DAPI, magnification ×200. Green: CK, blue: DAPI. (E) Correlation analysis of PCDHGA10 protein expression and tumor-infiltrating immune cells. (F) Correlation analysis of PCDHGA10 protein expression and immune checkpoints. DAPI: 4, 6-diamino-2-phenyl indole; CK, cytokeratin. * p < 0.05.
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FIGURE 4 Relationship between PCDHGA10 protein expression and tumor-infiltrating immune cells and immune checkpoints. (A-C) Multispectral composite of CD3, CD4, CD8, CD11b, CD66b, <t>CD68,</t> CK and DAPI, magnification ×200. (D) Four-color multispectral composite of LAG3, PD1, CK and DAPI, magnification ×200. Green: CK, blue: DAPI. (E) Correlation analysis of PCDHGA10 protein expression and tumor-infiltrating immune cells. (F) Correlation analysis of PCDHGA10 protein expression and immune checkpoints. DAPI: 4, 6-diamino-2-phenyl indole; CK, cytokeratin. * p < 0.05.
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FIGURE 4 Relationship between PCDHGA10 protein expression and tumor-infiltrating immune cells and immune checkpoints. (A-C) Multispectral composite of CD3, CD4, CD8, CD11b, CD66b, CD68, CK and DAPI, magnification ×200. (D) Four-color multispectral composite of LAG3, PD1, CK and DAPI, magnification ×200. Green: CK, blue: DAPI. (E) Correlation analysis of PCDHGA10 protein expression and tumor-infiltrating immune cells. (F) Correlation analysis of PCDHGA10 protein expression and immune checkpoints. DAPI: 4, 6-diamino-2-phenyl indole; CK, cytokeratin. * p < 0.05.

Journal: Frontiers in immunology

Article Title: PCDHGA10 as a potential prognostic biomarker and correlated with immune infiltration in gastric cancer.

doi: 10.3389/fimmu.2024.1500478

Figure Lengend Snippet: FIGURE 4 Relationship between PCDHGA10 protein expression and tumor-infiltrating immune cells and immune checkpoints. (A-C) Multispectral composite of CD3, CD4, CD8, CD11b, CD66b, CD68, CK and DAPI, magnification ×200. (D) Four-color multispectral composite of LAG3, PD1, CK and DAPI, magnification ×200. Green: CK, blue: DAPI. (E) Correlation analysis of PCDHGA10 protein expression and tumor-infiltrating immune cells. (F) Correlation analysis of PCDHGA10 protein expression and immune checkpoints. DAPI: 4, 6-diamino-2-phenyl indole; CK, cytokeratin. * p < 0.05.

Article Snippet: This study used the following primary antibodies: rabbit antiPCDHGA10 (1:50, D1247, Biobyt), rabbit anti-CD11b antibody (1:100, 49420S, CST), rabbit anti-CD8 antibody (1:100, ab83278, Frontiers in Immunology 03 Abcam), rabbit anti-CD3 antibody (1:200, 85061S, CST), rabbit anti-CD4 antibody (1:200, ab133616, Abcam), mouse anti-Foxp-3 antibody (1:50, ab20034, Abcam), anti-LAG-3 antibody (1:50, ab52587, Abcam), anti-CD66b antibody (1:500, ab214175, Abcam), anti-cytokeratin antibody (1:8000, orb69073, Biobyt), anti-CD68 antibody (1:500, 797778S, CST).

Techniques: Expressing